Identification and extraction of herbicidal compounds from metabolites of Trichoderma polysporum HZ-31

Abstract Trichoderma polysporum (Link) Rifai HZ-31 fermentation broth was separated and purified by extraction, column chromatography, and high-performance liquid chromatography. Four monomer compounds with strong herbicidal activity were obtained: p -hydroxyphenyl-2,3-dihydroxypropyl ether, o -hydroxy-3-carbonyl-1-phenylpropanol, 1,8-propanediol o -xylene, and 2-3-dihydroxypropyl propionate. The biological activity verification test indicated that the four monomer compounds could inhibit the germination of wild oat ( Avena fatua L.) and canola ( Brassica napus L.) seeds. Of the four, compound 3 (1,8-propanediol o -xylene) had obvious inhibitory effects on the germination of A. fatua and B. napus seeds, with inhibition rates of 83.33% and 86.67%, respectively. Therefore, the identification of this monomer compound lays a foundation for the further development of a novel microbial herbicide by directly utilizing it and developing new derivatives with herbicidal functions as lead compounds.