3P-348 : Novel affinity peptides for the use of influenza H5N1 Hemagglutinin (HA) detection identified by phage display screening

Hemagglutinin (HA) which derived from influenza virus H5N1 is a glycoprotein on surface of the virus. It has been used as antigen for diagnosis of influenza which causes seasonal epidemics of disease with high mortality rate. In this study, we isolated HA specific peptide motif (GHPHYNNPSLQL) by biopanning of random M13 phage library. After bipanning, we observed its binding affinity to HA by ELISA. Then, a series of synthetic peptides (IFA BP1 ~ IFA BP6) was chemically synthesized and immobilized on functionalized gold chips for detection of HA. The sensor performance was monitored by square wave voltammetry (SWV). Among six synthetic affinity peptides, IFA BP6 coated gold electrode had relatively high decreasing in current compared to other peptides. More details about the result will be talk in presentation.