Development of a comprehensive profiling method for regulatory lipid mediators using UPLC‐MS/MS

The regulatory lipid mediators are biologically active metabolites derived from polyunsaturated fatty acids such as the metabolites by cyclooxygenases, lipoxygenases, and cytochrome P450 enzymes. These pathways represent over 75% of pharmaceutical in mass in the world. By measuring endogenous metabolic responses to various environment stimulus, pathological processes and dietary supplements, lipidomics/metabolomics provide the most useful information about human responses. The knowledge gained from this type of research is very useful for many research fields including biomarker discovery, biotechnology advancement, discovery of novel biochemical mechanism, and assessment of clinical effectiveness. We developed a sensitive and comprehensive profiling method for the regulatory lipid mediators covering more than 150 lipid mediators including prostaglandins, leukotrienes, epoxy fatty acids, dihydroxy fatty acids, specialized pro‐resolving mediators, endocannabinoids, nitro‐fatty acids, poly‐unsaturated fatty acids and the trace for major species of phospholipids. Support or Funding Information This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .