吸附
寡核苷酸
DNA
解吸
化学
生物相容性
荧光
水溶液
组合化学
纳米技术
化学工程
生物物理学
材料科学
有机化学
生物化学
物理
工程类
生物
量子力学
作者
Zhaoqi Yang,Yue Qian,Fan Yang,Cheng Chen,Xiaosheng Tang,Jian Jin
出处
期刊:Langmuir
[American Chemical Society]
日期:2019-11-13
卷期号:35 (49): 16290-16296
被引量:24
标识
DOI:10.1021/acs.langmuir.9b02692
摘要
As an important subclass of MOFs, ZIF-8, built from 2-methylimidazole and Zn(NO3)2·6H2O, possesses excellent biocompatibility and high stability in aqueous solution. Recently, it has been found that ZIF-8 can efficiently adsorb DNA and quench the adsorbed fluorophores to a large extent. These properties make it possible to prepare DNA-based optical sensors using ZIF-8. Although practical analytical applications are being demonstrated, the basic understanding of the binding between ZIF-8 and DNA in solution has received relatively little attention. In this work, we report that the adsorption of 12-, 18-, 24-, and 36-mer single-stranded DNAs on ZIF-8 are affected by several factors. It is found from the outcomes that shorter DNAs are adsorbed more rapidly to the surface of ZIF-8. On the other hand, desorption of the probe DNA can be achieved using complementary strand DNA to restore the fluorescence value. Furthermore, the salt contributes to adsorption to some extent. These findings are important for further understanding of the interactions between DNA and ZIF-8 and for the optimization of DNA and MOF-based devices and sensors.
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