The inhabiting effect of Polygonum cuspidatum Sieb. et Zucc. on α-glucosidase.

[Objective]The research aimed to screen α-glucosidase inhibitor from Polygonum cuspidatum Sieb. et Zucc. . [Method]The inhabiting effect of Polygonum cuspidatum Sieb. et Zucc. extract on α-glucosidase was studied and the active ingredients were isolated. [Result]The resultsshowed that the inhibition rate of 50 mg/ml extract to α-glucosidase was 68.5%,its IC50 was about 37 mg/ml. The Polygonum cuspidatum Sieb. et Zucc. extract was noncompetitive inhibitor of α-glucosidase,and the Ki was 33 mg/L. The crude inhibitor was purified through DEAE-cellulose DE-52 and identified as polysaccharides. [Conclusion]The study provides basis for developing botanical α-glucosidase inhibitor and has an importante ffect on finding new safety medicine with high activity that can reduce postprandial plasma glucose.