脂质体
毕赤酵母
蛋白质组
细胞器
酵母
生物化学
内质网
酿酒酵母
生物
雅罗维亚
脂类学
微粒体
细胞生物学
化学
基因
酶
重组DNA
作者
Lisa Klug,Pablo Tarazona,Clemens Gruber,Karlheinz Grillitsch,Brigitte Gasser,Martin Trötzmüller,Harald Köfeler,Erich Leitner,Ivo Feußner,Diethard Mattanovich,Friedrich Altmann,Günther Daum
标识
DOI:10.1016/j.bbalip.2013.11.005
摘要
The methylotrophic yeast Pichia pastoris is a popular yeast expression system for the production of heterologous proteins in biotechnology. Interestingly, cell organelles which play an important role in this process have so far been insufficiently investigated. For this reason, we started a systematic approach to isolate and characterize organelles from P. pastoris. In this study, we present a procedure to isolate microsomal membranes at high purity. These samples represent endoplasmic reticulum (ER) fractions which were subjected to molecular analysis of lipids and proteins. Organelle lipidomics included a detailed analysis of glycerophospholipids, fatty acids, sterols and sphingolipids. The microsomal proteome analyzed by mass spectrometry identified typical proteins of the ER known from other cell types, especially Saccharomyces cerevisiae, but also a number of unassigned gene products. The lipidome and proteome analysis of P. pastoris microsomes are prerequisite for a better understanding of functions of this organelle and for modifying this compartment for biotechnological applications.
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