微泡
分泌物
细胞生物学
半乳糖凝集素
小泡
绿色荧光蛋白
半乳糖凝集素-3
外体
化学
单元格排序
融合蛋白
细胞
生物
生物化学
小RNA
免疫学
重组DNA
膜
基因
作者
Sebastian Bänfer,Sophie Kutscher,Ralf Jacob
出处
期刊:Springer eBooks
[Springer Nature]
日期:2022-01-01
卷期号:: 413-424
被引量:1
标识
DOI:10.1007/978-1-0716-2055-7_22
摘要
Cells use unconventional secretion to deliver the β-galactoside binding lectin galectin-3 from the cell interior into the extracellular milieu. This process starts with galectin-3 recruitment into intraluminal vesicles (ILVs), which are later released at the plasma membrane as exosomes. Electron microscopy is utilized to determine the location of GFP-tagged galectin-3 in pelleted exosomes. We also describe how these vesicles are harvested from cell culture media to determine their composition. The fluorescent protein GFP was fused with the exosomal sorting motif of galectin-3 to direct GFP into exosomes. Recruitment of this fusion construct into the lumen of exosomes can be assessed by proteinase K accessibility analysis.
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