Purine nucleoside phosphorylases obtained from Er. carotovora and Cor. sepedonicum were partially purified and some properties of these enzymes were studied. Purine nucleoside phosphorylases obtained from Er. carotovora and Cor. sepedonicum catalyzed the formation of inosine, guanosine, adenosine and xanthosine, though the reaction rate was different with each enzyme.