DNA Origami as a Nanoscopic Ruler for Super‐Resolution Microscopy

Resolving the distances: Rectangular DNA origami labeled with fluorophores at specific positions has been used as a nanoscopic ruler. Super-resolution microscopy based on the subsequent localization of single molecules enables two fluorophores at a distance of about 90 nm to be optically resolved. This combination of subdiffraction imaging and DNA nanotechnology opens up new avenues for studying nanostructures and their dynamics. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.