化学
电迁移
毛细管电泳
色谱法
分离法
样品制备
纳米技术
复合材料
材料科学
作者
Sille Štěpánová,Václav Kašička
标识
DOI:10.1016/j.aca.2022.339447
摘要
This paper provides a detailed overview of relevant developments in separation and analysis of proteins by capillary electromigration (CE) methods in the period 2017-mid 2021. The presented topics embrace sample preparation, suppression of protein sorption, control of electroosmotic flow, separations of proteins by the particular CE methods and protein identification and quantification by various detection techniques. To illustrate the wide usability of CE methods for protein analysis, the paper shows several novel applications of CE methods, such as quality control and characterization of protein biopharmaceuticals, determination of proteins in complex biomatrices, investigation of posttranslational modification, peptide mapping, interactions of proteins with other (bio)molecules and determination of their physicochemical and biochemical characteristics.
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