肾透明细胞癌
癌症研究
生物
转录因子
癌基因
基因沉默
癌症
异位表达
河马信号通路
细胞生长
细胞周期
细胞培养
肿瘤科
医学
肾细胞癌
基因
遗传学
作者
Fang Li,Yun Feng,Qingwei Jiang,Jinyuan Zhang,Fei Wu,Qian Li,Xinli Jing,Xiaofei Wang,Chen Huang
出处
期刊:Life Sciences
[Elsevier]
日期:2022-03-01
卷期号:293: 120327-120327
被引量:8
标识
DOI:10.1016/j.lfs.2022.120327
摘要
Transcriptional enhanced associate domain (TEAD) transcription factor family, a very important family in the hippo signaling pathway, has been found to play oncogenic functions in the occurrence of various malignant tumors. However, the expression of TEADs in pan-cancer and the important role of TEAD4 in clear cell renal cell carcinoma (ccRCC) have not been analyzed. Herein, we aim to evaluate the expression of TEADs in pan-cancer, and focus on analyzing the role of TEAD4 in the progression of ccRCC.Data from the Cancer Genome Atlas (TCGA) was used to analyze the expression of TEADs in pan-cancer and its clinical correlation. TEAD4 expression in ccRCC tissues, biological functions in vitro and in vivo were analyzed by immunohistochemistry (IHC), western blotting, RNAi and Xenograft assay. Mircode, BioGRID and g: Profiler website were used to build a ceRNA network and downstream pathway prediction.TEAD1, TEAD2, TEAD3 and TEAD4 were highly expressed in 3, 6, 5, and 12 types of cancer tissues, respectively, indicating that TEAD4 is most closely related to tumor progression. Among the cancers with high TEAD4 expression, the expression of TEAD4 has the greatest correlation with the poor prognosis of ccRCC. We also found the malignant phenotypes of ccRCC cells in vitro and vivo have been significantly suppressed by silencing TEAD4.TEADs, especially TEAD4, were overexpressed in many human tumors. This study is the first to show that TEAD4 acts as an oncogene in ccRCC and may be an important factor in progress of ccRCC.
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