自愈水凝胶
壳聚糖
聚己内酯
复合数
活力测定
化学
MTT法
磷灰石
抗菌活性
表皮葡萄球菌
伤口愈合
材料科学
化学工程
核化学
高分子化学
复合材料
金黄色葡萄球菌
细胞
有机化学
生物化学
细菌
医学
外科
矿物学
遗传学
生物
工程类
聚合物
作者
Natjaya Ekapakul,Chomdao Sinthuvanich,Hiroharu Ajiro,Chantiga Choochottiros
标识
DOI:10.1016/j.ijbiomac.2022.05.139
摘要
Recently, our group reported the synthesis and fabrication of composite hydrogels of chitosan (CS) and star-shaped polycaprolactone (stPCL). The co-crosslink of modified stPCL with carboxyl at the end chain (stPCL-COOH) provided good mechanical properties and stability to the composite hydrogels. This research presents the bioactivities of composite hydrogels showing a potential candidate to develop biomaterials such as wound dressing and bone tissue engineering. The bioactivities were the antibacterial activity, cell viability, skin irritation, decomposability, and ability to attach ions for apatite nucleation. The results showed that all the composite hydrogels were completely decomposed within 2 days. The composite hydrogels had better antibacterial activity and higher efficiency to Gram-negative (Escherichia coli) than to Gram-positive (Staphylococcus epidermidis) bacteria. The composite hydrogels were studied for cell viability based on MTT assay and skin irritation on rabbit skin. The results indicated high cell survival more than 80% and no skin irritation. In addition, the results showed that calcium and phosphorous were preferentially attached to the composite hydrogel surface to grow apatite crystal (Ca/P ratio 1.86) compared to attaching to the chitosan hydrogel (Ca/P ratio 1.48) in 21 days of testing.
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