肺纤维化
ATG12
衰老
人参皂苷Rg1
自噬
百草枯
细胞凋亡
细胞生物学
特发性肺纤维化
转染
人参
纤维化
化学
肺
生物
细胞培养
人参皂甙
医学
生物化学
病理
内科学
ATG5型
遗传学
替代医学
作者
Changbao Huang,Xiang Xue,Nengkai Gong,Jinghan Jiang
摘要
Abstract Paraquat (PQ), as a widely used herbicide, is highly toxic to human. PQ‐induced pulmonary fibrosis is the main reason for respiratory failure and death. In PQ‐poisoned mice, we find abundant senescent epithelial cells in the lung tissues, which can contribute to the activation of pulmonary fibroblasts. Ginsenoside Rg1 (Rg1), the main active component of ginseng, possess beneficial properties against aging. In our work, we aimed to investigate the potential protective effects of Rg1 on PQ‐induced pulmonary fibrosis and the underlying mechanism. In vivo, the treatment of Rg1 can attenuate PQ‐induced pulmonary fibrosis and decrease senescence and senescence associated secretory phenotype (SASP) expression. In vitro, Rg1 can effectively eliminate senescent cells via apoptosis, but not normal cells. In addition, we demonstrate that Rg1 can enhance autophagy activity via inducing the expression of ATG12. Inhibition of autophagy via 3‐MA or transfection of the siRNA targeting ATG12 can impair the antiaging effect of Rg1. Taken together, our data implicates that Rg1 can protect pulmonary epithelial cells from PQ‐induced cellular senescence in an ATG12 dependent manner, which may provide a preventive and therapeutic strategy for PQ poisoning‐induced pulmonary fibrosis.
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