荟萃分析
系统回顾
背景(考古学)
肿瘤坏死因子α
医学
炎症反应
原始分数
炎症
免疫学
内科学
梅德林
化学
原始数据
计算机科学
生物
生物化学
古生物学
程序设计语言
作者
B. Facchin,Gustavo Oliveira dos Reis,Guilherme Nicácio Vieira,Eduarda Talita Bramorski Mohr,Júlia Salvan da Rosa,Iara Fabrícia Kretzer,Izabel Galhardo Demarchi,Eduardo Monguilhott Dalmarco
标识
DOI:10.1007/s00011-022-01584-0
摘要
IntroductionSeveral experimental models have been designed to promote the development of new anti-inflammatory drugs. The in vitro model using RAW 264.7 cells has been widely used. However, there is still no consensus on which inflammatory mediators should initially be measured to screen for possible anti-inflammatory effects. To determine the rationality of measuring inflammatory mediators together with NO, such as the levels of tumor necrosis factor (TNF)-α, and interleukins (IL) 1β and 6, we carried out this systematic review (SR) and meta-analysis (MA).MethodologyWe conducted this SR and MA in accordance with the Preferred Reporting of Systematic Reviews and Meta-Analysis and the Cochrane Handbook for Systematic Reviews of Intervention. This review was registered in the Open Science Framework (https://doi.org/10.17605/OSF.IO/8C3HT).ResultsLPS-induced cells produced high NO levels compared to non-LPS induced, and this production was not related to cell density. TNF-α, IL-1β, and IL-6, also showed high levels after cells had been stimulated with LPS. Though with some restrictions, all studies were reliable, as the risk of bias was detected in the test compounds and systems.ConclusionMeasurement of NO levels may be sufficient to screen for possible anti-inflammatory action in the context of LPS-induced RAW 264.7 cells.
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