基因敲除
染色质免疫沉淀
下调和上调
长非编码RNA
转录因子
维甲酸
Ccaat增强子结合蛋白
急性早幼粒细胞白血病
抄写(语言学)
癌症研究
生物
细胞生长
分子生物学
细胞生物学
化学
发起人
细胞培养
基因表达
基因
核蛋白
生物化学
遗传学
哲学
语言学
作者
Yewei Wang,Ling Fu,Ailian Sun,Doudou Tang,Yadong Xu,Zheyuan Li,Mingjie Chen,Guangsen Zhang
标识
DOI:10.1016/j.bbrc.2017.10.137
摘要
Emerging evidences have shown that long non-coding RNAs (lncRNAs) play critical roles in cancer development and cancer therapy. LncRNA Nuclear Enriched Abundant Transcript 1 (NEAT1) is indispensable during acute promyelocytic leukemia (APL) cell differentiation induced by all-trans retinoic acid (ATRA). However, the precise mechanism of NEAT1 upregulation has not been fully understood. In this study, we performed chromatin immunoprecipitation and luciferase reporter assays to demonstrate that C/EBP family transcription factor C/EBPβ bind to and transactivate the promoter of lncRNA NEAT1 through the C/EBPβ binding sites both around −54 bp and −1453 bp upstream of the transcription start site. Moreover, the expression of C/EBPβ was increased after ATRA treatment, and the binding of C/EBPβ in the NEAT1 promoter was also dramatically increased. Finally, knockdown of C/EBPβ significantly reduced the ATRA-induced upregulation of NEAT1. In conclusion, C/EBPβ directly activates the expression of NEAT1 through binding to the promoter of NEAT1. Knockdown of C/EBPβ impairs ATRA-induced transcriptional activation of NEAT1. Our data indicate that C/EBPβ contributes to ATRA-induced activation of NEAT1 during APL cell differentiation. Our results enrich our knowledge on the regulation of lncRNAs and the regulatory role of C/EBPβ in APL cell differentiation.
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