拉链
心理压抑
原癌基因蛋白质c-myc
癌变
细胞生物学
细胞凋亡
亮氨酸拉链
转录因子
生物
螺旋
基因
C2C12型
分子生物学
化学
基因表达
DNA结合蛋白
遗传学
计算机科学
算法
肌发生
作者
Laura Soucek,Richard Jucker,Laura Panacchia,R. Ricordy,Franco Tatò,Sergio Nasi
出处
期刊:PubMed
日期:2002-06-15
卷期号:62 (12): 3507-10
被引量:59
摘要
The Myc basic helix-loop-helix zipper domain determines dimerization with Max and binding to the DNA E-box, both of which play a critical role in Myc regulation of growth, proliferation, tumorigenesis, and apoptosis. The mutant basic helix-loop-helix zipper domain, Omomyc, dimerizes with Myc, sequestering it in complexes unable to bind the E-box, and so acting as a potential dominant negative. Consistent with this, Omomyc reverses Myc-induced cytoskeletal disorganization in C2C12 myoblasts. Surprisingly, however, Omomyc strongly potentiates Myc-induced apoptosis in a manner dependent on Myc expression level. Expression analysis of known Myc target genes indicates that Omomyc inhibits transcriptional activation but enhances repression. These findings suggest that Omomyc can selectively trigger apoptosis in cells overexpressing Myc, possibly through the transcriptional repression of specific genes.
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