医学微生物学
流行病学
聚合酶链反应
多重聚合酶链反应
病毒学
医学
生物
呼吸系统
内科学
生物化学
基因
作者
David Lieberman,Avi Shimoni,Ayelet Keren-Naus,Rosalie Steinberg,Yonat Shemer‐Avni
标识
DOI:10.1007/s10096-010-0903-5
摘要
A pooled sample of oropharyngeal swabs, nasopharyngeal swabs and nasopharyngeal washings, taken from each of 1,000 subjects, was compared to separate specimens from the same sampling. Multiplex real-time polymerase chain reaction (mqRT-PCR) was used to identify 12 respiratory viruses. Two hundred and forty-three (97%) of the 251 viruses identified in the separate samples were also identified in the mixed samples. The sensitivity rate was identical at 100% for all virus groups except coronaviruses. This sensitivity rate clearly justifies the use of pooled samples instead of separate samples for clinical and epidemiological purposes. The reduction in costs attained from the use of pooled samples may represent a critical advantage when considering its use in extensive clinical and epidemiological studies.
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