Enzymic Disruption of N‐Aroyl‐L‐homoserine Lactone‐Based Quorum Sensing

Quorum-sensing molecules: A genetically encodable metallo-γ-lactonase from B. thuringiensis (AiiA) is shown to effectively quench N-aroyl-L-homoserine lactone-based signaling. Here it is reported that N-aroyl-HSLs are excellent substrates of AiiA, and that the broad substrate specificity of AiiA makes it a useful tool in the quenching of a wide variety of naturally occurring and coumaroyl-L-homoserine lactones (pC-HSLs).