Leptatriose (2), a novel trisaccharide, was obtained from acid hydrolysate of Leptaculatin (1), isolated from Leptadenia reticulata. On the basis of physico-chemical results, the structure of 2 was established as O-β-d-glucopyranosyl (1>4)-O-β-d-glucopyranosyl (1>4)-β-d-cymaropyranose.