Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method

Abstract Brazilian plant extracts belonging to 16 species of 5 different families (71 extracts) were tested against the stable DPPH (2,2‐diphenyl‐1‐picryl‐hydrazyl‐hydrate) free‐radical. The ability to scavenge DPPH radical was measured in these experiments by the discoloration of the solution. Ginkgo biloba and rutin, commonly used as antioxidants for medical purposes, were used as standards. Based on our results, we can say that as a general rule the ethanol extracts of plants belonging to the Verbenaceae family showed lower EC 50 values than the other plant extracts. Among the partitions, the more polar ones (ethyl acetate and n ‐butanol) are those that generally have higher antioxidant activity (AA). Copyright © 2001 John Wiley & Sons, Ltd.