SIRT3-mediated mitophagy by deacetylating ATP5F1A involved in the protective effects of SIGMAR1/Sigma-1 receptor against ferroptosis and microvascular hyperpermeability in lipopolysaccharide-induced acute lung injury

: ALI, acute lung injury; ARDS, acute respiratory distress syndrome; ATP, adenosine triphosphate; ATP5F1A, ATP synthase F1 subunit alpha; BCA, bicinchoninic acid; EB, Evans blue dye; ECM, endothelial cell medium; FBS, fetal bovine serum; FITC, fluorescein isothiocyanate; Fer-1, ferrostatin-1; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; GFP-LC3, green fluorescent protein-microtubule associated protein 1 light chain 3 alpha; GPX4, glutathione peroxidase 4; GSH, glutathione; GSSG, glutathione disulfide; KO, knockout; LPS, lipopolysaccharide; LRRK2, leucine rich repeat kinase 2; MDA, malondialdehyde; MPMVECs, mouse pulmonary microvascular endothelial cells; MTT, 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide; PBS, phosphate-buffered saline; PECAM1/CD31, platelet and endothelial cell adhesion molecule 1; PRKN, parkin RBR E3 uniquitin protein ligase; ROS, reactive oxygen species; RSL3, RAS-selective lethal 3; SDS-PAGE, sodium dodecyl sulfate-polyacrylamide gel electrophoresis; SIGMAR1, sigma non-opioid intracellular receptor 1; SIRT3, sirtuin 3; siRNA, small interfering RNA; TUNEL, terminal deoxyribonucleotidyl transferase-mediated deoxyuridine 5-triphosphate-digoxigenin nick end labeling; VHP, vascular hyperpermeability; W:D, wet:dry; WT, wild type.