光老化
姜黄
化学
根茎
抗氧化剂
H&E染色
人体皮肤
乙醇
姜黄素
免疫组织化学
传统医学
药理学
皮肤病科
生物化学
医学
病理
生物
遗传学
作者
Yating Zheng,Chunxing Pan,Zejun Zhang,Luo Wen-qian,Xinmiao Liang,Yaohui Shi,Linjie Liang,Xi Zheng,Lanyue Zhang,Zhiyun Du
标识
DOI:10.1016/j.microc.2020.104608
摘要
Essential oil derived from the rhizome of Curcumin longa (CL-EO) has marked anti-inflammatory, antioxidant, antimicrobial, anticancer, and antiviral activities. However, its effect on photodamaged skin has not yet been evaluated. In this study, the anti-skin photoaging activity of CL-EO was determined by performing an ultraviolet B (UVB)-induced skin aging assay. CL-EO was extracted by hydrodistillation and characterized using gas chromatography–mass spectrometry. The anti-skin aging effect was determined by topically applying 150 μl of CL-EO diluted to 1, 5, and 10% with ethanol to the dorsal area of UVB-irradiated nude mice every day except on Sunday for 8 weeks. Histological and immunohistochemical analyses were performed. In total, 56 compounds, which accounted for 94.36% of the contents of CL-EO, were detected. The major compounds in CL-EO were ar-turmerone (36.04%), curlone (8.78%), β-turmerone (7.05%), 8,9-dehydro-9-formyl-cycloisolongifolene (5.69%), β-sesquiphellandrene (5.39%), germacrone (4.51%), ar-curcumene (2.19%), α-himachalene (2.14%), and ledane (2.13%). Hematoxylin and eosin staining of tissue sections revealed that CL-EO decreased epidermal skin thickness. Immunohistochemistry showed that CL-EO inhibited interleukin-1β and tumor necrosis factor-α expression. Thus, CL-EO can reduce cutaneous photoaging in a UVB-irradiated nude mouse model. Therefore, CL-EO could be used in the formulation of skin care and functional cosmetic products.
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