化学
硝化棉
多菌灵
检出限
分析物
灵敏度(控制系统)
聚乙烯醇
色谱法
杀菌剂
膜
植物
电子工程
工程类
生物
生物化学
有机化学
作者
Pumnatthiga Makkala,Sirowan Ruantip,Anumart Buakeaw,Sudkate Chaiyo,Nanthika Khongchareonporn
出处
期刊:Talanta
[Elsevier]
日期:2024-03-01
卷期号:: 125911-125911
标识
DOI:10.1016/j.talanta.2024.125911
摘要
Lateral flow immunoassays (LFIAs) are widely used to determine carbendazim (CBZ) residues in food products due to their advantages of low cost, ease and rapid use, on-site detection capability. However, conventional LFIAs have low detection sensitivity. Although improvements have been made to increase the sensitivity, it is not sufficient. Here, a hamper pad, polyvinyl alcohol coated on a nitrocellulose membrane, was integrated to enhance the sensitivity of LFIA for CBZ detection. The hamper pad was inserted between the conjugated and nitrocellulose pads to delay the flow rate, thereby increasing the possibility of the antibody and target analyte binding. This platform exhibited a fourfold sensitivity increase in CBZ detection compared with the conventional LFIA, and its limit of detection was 1.6 ng/mL. In addition, a single-step operation was successfully applied to detect CBZ in rice (white rice, brown rice, sticky rice, and paddy) and soybean samples, with acceptable recoveries of 93.6%-120.0%. This novel device was compared to the standard high-performance liquid chromatography method, which shows high accuracy with a Kappa coefficient of 0.91. Therefore, improved sensitivity with a rapid, simple, and inexpensive device could facilitate the detection of CBZ residues in agricultural products for on-field screening and improved user-friendliness.
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