Highly Multiplexed, Efficient, and Automated Single‐Cell MicroRNA Sequencing with Digital Microfluidics. (Small Methods 3/2024)

数字微流体 微流控 多路复用 小RNA 计算生物学 计算机科学 数字聚合酶链反应 纳米技术 生物 化学 材料科学 遗传学 基因 电信 聚合酶链反应 电润湿 物理化学 电极
作者
Yingwen Chen,Xuanqun Wang,Na Xing,Yingkun Zhang,Zan Li,Xiaohong Chen,Linfeng Cai,Jia Song,Ren Xu,Chaoyong Yang
出处
期刊:Small methods [Wiley]
卷期号:8 (3)
标识
DOI:10.1002/smtd.202470011
摘要

Small MethodsVolume 8, Issue 3 2470011 Cover PictureFree Access Highly Multiplexed, Efficient, and Automated Single-Cell MicroRNA Sequencing with Digital Microfluidics. (Small Methods 3/2024) Yingwen Chen, Yingwen Chen State Key Laboratory for Physical Chemistry of Solid Surfaces, Key Laboratory for Chemical Biology of Fujian Province, Key Laboratory of Analytical Chemistry, and Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005 ChinaSearch for more papers by this authorXuanqun Wang, Xuanqun Wang State Key Laboratory for Physical Chemistry of Solid Surfaces, Key Laboratory for Chemical Biology of Fujian Province, Key Laboratory of Analytical Chemistry, and Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005 ChinaSearch for more papers by this authorXing Na, Xing Na State Key Laboratory for Physical Chemistry of Solid Surfaces, Key Laboratory for Chemical Biology of Fujian Province, Key Laboratory of Analytical Chemistry, and Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005 ChinaSearch for more papers by this authorYingkun Zhang, Yingkun Zhang State Key Laboratory for Physical Chemistry of Solid Surfaces, Key Laboratory for Chemical Biology of Fujian Province, Key Laboratory of Analytical Chemistry, and Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005 ChinaSearch for more papers by this authorZan Li, Zan Li Department of Sports Medicine, Xiangya Hospital, Central South University, Changsha, 410008 ChinaSearch for more papers by this authorXiaohui Chen, Xiaohui Chen State Key Laboratory of Cellular Stress Biology, The First Affiliated Hospital of Xiamen University-ICMRS Collaborating Center for Skeletal Stem Cell, School of Medicine, Xiamen University, Xiamen, 361100 China Fujian Provincial Key Laboratory of Organ and Tissue Regeneration, School of Medicine, Xiamen University, Xiamen, 361100 ChinaSearch for more papers by this authorLinfeng Cai, Linfeng Cai State Key Laboratory for Physical Chemistry of Solid Surfaces, Key Laboratory for Chemical Biology of Fujian Province, Key Laboratory of Analytical Chemistry, and Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005 ChinaSearch for more papers by this authorJia Song, Jia Song Institute of Molecular Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127 ChinaSearch for more papers by this authorRen Xu, Ren Xu State Key Laboratory of Cellular Stress Biology, The First Affiliated Hospital of Xiamen University-ICMRS Collaborating Center for Skeletal Stem Cell, School of Medicine, Xiamen University, Xiamen, 361100 China Fujian Provincial Key Laboratory of Organ and Tissue Regeneration, School of Medicine, Xiamen University, Xiamen, 361100 ChinaSearch for more papers by this authorChaoyong Yang, Chaoyong Yang State Key Laboratory for Physical Chemistry of Solid Surfaces, Key Laboratory for Chemical Biology of Fujian Province, Key Laboratory of Analytical Chemistry, and Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005 China Institute of Molecular Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127 ChinaSearch for more papers by this author Yingwen Chen, Yingwen Chen State Key Laboratory for Physical Chemistry of Solid Surfaces, Key Laboratory for Chemical Biology of Fujian Province, Key Laboratory of Analytical Chemistry, and Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005 ChinaSearch for more papers by this authorXuanqun Wang, Xuanqun Wang State Key Laboratory for Physical Chemistry of Solid Surfaces, Key Laboratory for Chemical Biology of Fujian Province, Key Laboratory of Analytical Chemistry, and Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005 ChinaSearch for more papers by this authorXing Na, Xing Na State Key Laboratory for Physical Chemistry of Solid Surfaces, Key Laboratory for Chemical Biology of Fujian Province, Key Laboratory of Analytical Chemistry, and Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005 ChinaSearch for more papers by this authorYingkun Zhang, Yingkun Zhang State Key Laboratory for Physical Chemistry of Solid Surfaces, Key Laboratory for Chemical Biology of Fujian Province, Key Laboratory of Analytical Chemistry, and Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005 ChinaSearch for more papers by this authorZan Li, Zan Li Department of Sports Medicine, Xiangya Hospital, Central South University, Changsha, 410008 ChinaSearch for more papers by this authorXiaohui Chen, Xiaohui Chen State Key Laboratory of Cellular Stress Biology, The First Affiliated Hospital of Xiamen University-ICMRS Collaborating Center for Skeletal Stem Cell, School of Medicine, Xiamen University, Xiamen, 361100 China Fujian Provincial Key Laboratory of Organ and Tissue Regeneration, School of Medicine, Xiamen University, Xiamen, 361100 ChinaSearch for more papers by this authorLinfeng Cai, Linfeng Cai State Key Laboratory for Physical Chemistry of Solid Surfaces, Key Laboratory for Chemical Biology of Fujian Province, Key Laboratory of Analytical Chemistry, and Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005 ChinaSearch for more papers by this authorJia Song, Jia Song Institute of Molecular Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127 ChinaSearch for more papers by this authorRen Xu, Ren Xu State Key Laboratory of Cellular Stress Biology, The First Affiliated Hospital of Xiamen University-ICMRS Collaborating Center for Skeletal Stem Cell, School of Medicine, Xiamen University, Xiamen, 361100 China Fujian Provincial Key Laboratory of Organ and Tissue Regeneration, School of Medicine, Xiamen University, Xiamen, 361100 ChinaSearch for more papers by this authorChaoyong Yang, Chaoyong Yang State Key Laboratory for Physical Chemistry of Solid Surfaces, Key Laboratory for Chemical Biology of Fujian Province, Key Laboratory of Analytical Chemistry, and Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005 China Institute of Molecular Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127 ChinaSearch for more papers by this author First published: 20 March 2024 https://doi.org/10.1002/smtd.202470011AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Graphical Abstract Front Cover In article number 2301250, Xu, Yang, and co-workers introduce a highly multiplexed, efficient, integrated, and automated sample preparation platform for single-cell miRNA sequencing based on digital microfluidics, where multiple single cells can be selectively isolated and subject to sample processing in a highly parallel way. Volume8, Issue3March 20, 20242470011 RelatedInformation

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