Pore Size Tunable Trypsin@ZIF-90 and Hydrogel Integrated Lateral Flow Point-of-Care Platform for ATP Detection

The cost-effective, convenient, visible, and equipment-free determination of biomarkers is always the priority development concern of disease diagnosis. The paper-based signal output strategy permits output visual signals without instruments and is regarded as a promising approach with simple operation and low cost. Herein, by varying the addition amount of trypsin, we pioneered a novel enzyme mineralization strategy to construct trypsin@ZIF-90 with tunable porosity properties and catalytic activity. The successful synthesis of trypsin@ZIF-90, which is tagged with T 1, T 3,... (T x, x is the addition amount of trypsin. Unit: mg), demonstrated the feasibility of this strategy. By serving the constructed trypsin@ZIF-90-T 1 as the target recognition module, and a new designed hydrogel-integrated pH indicator strip as the signal reporter, a point-of-care test (POCT) platform was developed for convenient and equipment-free measurement of adenosine triphosphate (ATP). The enzymatic activity measurement of trypsin@ZIF-90 and concurrently the quantitative analysis of ATP can be favorably realized by simple counting the flow distance and coverage area of water released during the reaction on a pH indicator strip. As a result, this portable platform can enable rapid detection of ATP in the linear range of 20–1500 μM and possesses favorable sensitivity, selectivity, and applicability. Thus, the constructions of tunable frameworks and paper-based POCT are of outstanding significance in the fields of porous metal–organic framework synthesis, enzyme mineralization, and rapid detection for medical diagnostics and environmental monitoring applications.