A novel yolk-shell nanoparticles delivery system based on Polygonatum polysaccharides and PCC1 for synergistic anti-aging

The activity of procyanidin C1 (PCC1) is limited by poor intestinal targeting, while traditional delivery systems typically neglect the adjuvant activity of the wall material. This study aimed to explore the potential of Polygonatum polysaccharides (PSP) as wall materials for delivery systems with synergistic effects. A novel PCC1 yolk-shell nanoparticles delivery system (PCC1-P/L NPs) were fabricated using lysine as a cross-linking agent, achieving a drug loading efficiency of 93.57 %. Molecular docking analysis revealed that PSP interacted with PCC1 and lysine through hydrogen bonds and hydrophobic interactions. We further evaluated the targeted delivery efficiency and synergistic effect of PCC1-P/L NPs. It significantly increased the PCC1 concentrations in the intestine by twofold. Furthermore, PCC1-P/L NPs exerted a synergistic anti-aging effect, extending the maximum lifespan of Drosophila by 30.8 %, which significantly exceeded the effect of PCC1 or PSP alone. Moreover, the delivery system potently suppressed the accumulation of pH 3+ and esg + cells and preserved gut integrity. This study highlights PSP as a multifunctional wall material and offers a new strategy for anti-aging.