适体
检出限
化学
亚甲蓝
电化学
电化学气体传感器
污染物
组合化学
纳米技术
核化学
色谱法
材料科学
电极
分子生物学
生物
生物化学
有机化学
催化作用
光催化
物理化学
作者
Yue Wang,Hongguo Zhai,Qi Guo,Yuhao Zhang,Xia Sun,Yemin Guo,Qingqing Yang,Yanyan Zhang
标识
DOI:10.1016/j.snb.2023.134648
摘要
Heavy metals and foodborne pathogens have become key pollutants that harm aquatic products, seriously endangering food safety and human health. Therefore, in this study, a ratiometric electrochemical aptamer sensor with a novel signal amplification strategy was constructed using ZrO2 as the substrate and Ni/Co-MOFs@AuNPs cubic nanomaterials to increase the specific surface area to simultaneously detect Cd2+ and S.aureus. The shared hairpin complementary chains modified with ferropene (Fc) could simultaneously bind to the Cd2+ and S.aureus aptamers modified with methylene blue (MB) and anthrquinone-2-carboxylic acid (AQ). The ratios of MB and AQ to Fc were used to realize the qualitative and quantitative detection of Cd2+ and S.aureus. Under the optimal conditions, the electrochemical aptamer sensor for Cd2+ and S.aureus was in the range of 1.00 × 10−2-1.00 × 104 ng/L and 1.00 × 101-1.00 × 107 CFU/mL, and the lowest detection limit was 1.91 × 10−4 ng/L and 1.37 CFU/mL, respectively. The aptasensor constructed in this paper can realize the simultaneous detection of Cd2+ and S.aureus in scallop and fish, providing a new detection method for the simultaneous detection of a variety of aquatic products pollutants.
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