OLIGOSACCHARIDE ESTERS AND XANTHONES FROM ROOTS OF POLYGALA HYBRIDA AND POLYGALA SIBIRICA (POLYGALACEAE)

Genus Polygala Tourn. ex L. is represented in Siberia by three species including two poorly understood species, P. hybrida DC. (syn. P. comosa subsp. comosa, P. comosa var. hybrida (DC.) Petelin) and P. sibirica L. which roots are used in Eastern medicine. Chromatographic separation of P. hydrida roots components led to isolation of 21 substances identified by UV, NMR spectroscopy and mass spectrometry as xanthone glycosides (lancerin, polygalaxanthones III, VII, XI, sibiricaxanthone B, 7-methoxymangiferin) and aglycone (6-hydroxy-1,2,3,7-tetramethoxyxanthone) and oligosaccharide esters (sibericose A4, A5, A6, 6,3′-di-O-sinapoyl sucrose, 6-O-sinapoyl-3′-O-feruloyl sucrose, tenuifoliside C, tenuifolioses A, B, D, H, I, J). Two new natural compounds were isolated ana described as disubstituted hydroxycinnamoyl esters of sucrose, 4-O-sinapoyl-3′-O-3,4,5-trimethoxycinnamoyl sucrose (isotenuifoliside C1) and 4-O-3,4,5-trimethoxycinnamoyl-3′-O-sinapoyl sucrose (isotenuifoliside C2). Application of the liquid chromatography-mass spectrometry revealed the presence of 24 components in P. sibirica roots and 17 of them for the first time for the species. Quantitative analysis indicated a high content of oligosaccharide esters and xanthones in the roots of P. hydrida (56.19 and 11.64 mg/g, respectively) and P. sibirica (64.82 and 9.72 mg/g, respectively) indicating the prospects for the further reseach of two studied Polygala species.