清晨好,您是今天最早来到科研通的研友!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您科研之路漫漫前行!

[Integrated transcriptomics and metabolomics analysis of flavonoid biosynthesis in

代谢组学 类黄酮 转录组 类黄酮生物合成 生物合成 化学 计算生物学 生物化学 生物 色谱法 基因表达 基因 抗氧化剂
作者
Song Gao,Min Qiu,Xiu‐Qing Li,Qian Zhao,Erli Niu
出处
期刊:PubMed 卷期号:41 (2): 588-601
标识
DOI:10.13345/j.cjb.240655
摘要

Ophiopogon japonicus, a precious medicinal plant endemic to Zhejiang Province. Its tuberous roots are rich in bioactive components such as flavonoids, possessing anti-inflammatory, antioxidant, and immunomodulatory properties. To elucidate the impact of cadmium (Cd) stress on the accumulation and biosynthetic pathway of flavonoids in O. japonicus, this study exposed O. japonicus to different concentrations of Cd stress and explored the changes through integrated transcriptomics and metabolomics analysis. The results demonstrated that Cd stress (1 mg/L and 10 mg/L) significantly increased the content of flavonoids in O. japonicus in a concentration-dependent manner. The metabolomics analysis revealed a total of 110 flavonoids including flavones, flavanols, flavonols, flavone and flavonol derivatives, flavanones, isoflavonoids, chalcones and dihydrochalcones, and anthocyanins in O. japonicus, among which flavones, flavonols, flavone and flavonol derivatives, and anthocyanins increased under Cd stress. The transcriptomics analysis identified several key flavonoid biosynthesis-associated genes with up-regulated expression under Cd stress, including 14 genes encoding 4-coumarate CoA ligase (4CL), 2 genes encoding chalcone isomerase (CHI), and 14 genes encoding phenylalanine ammonia lyase (PAL). The gene-metabolite regulatory network indicated significant positive correlations of 4CL (Cluster-21637.5012, Cluster-21637.90648, and Cluster-21637.62637) and CHI (Cluster-21637.111909 and Cluster-21637.123300) with flavonoid metabolites, suggesting that these genes promoted the synthesis of specific flavonoid metabolites, which led to the accumulation of total flavonoids under Cd stress. These findings provide theoretical support for the cultivation and utilization of medicinal plants in Cd-contaminated environments and offered new perspectives for studying plant responses to heavy metal stress.

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
Owen应助科研通管家采纳,获得10
19秒前
斯文败类应助卡卡采纳,获得10
22秒前
量子星尘发布了新的文献求助10
24秒前
25秒前
29秒前
整齐代真发布了新的文献求助10
32秒前
36秒前
Shawn完成签到 ,获得积分10
49秒前
量子星尘发布了新的文献求助30
51秒前
赵女士完成签到,获得积分10
51秒前
Mountain发布了新的文献求助10
1分钟前
Mountain完成签到,获得积分10
1分钟前
1分钟前
量子星尘发布了新的文献求助10
1分钟前
熊十九完成签到,获得积分10
1分钟前
1分钟前
1分钟前
2分钟前
2分钟前
量子星尘发布了新的文献求助10
2分钟前
坦率的语芙完成签到,获得积分10
2分钟前
量子星尘发布了新的文献求助10
2分钟前
量子星尘发布了新的文献求助10
3分钟前
3分钟前
Star发布了新的文献求助10
3分钟前
量子星尘发布了新的文献求助10
3分钟前
田様应助勇往直前采纳,获得10
3分钟前
Star完成签到,获得积分10
3分钟前
深情安青应助科研通管家采纳,获得10
4分钟前
4分钟前
量子星尘发布了新的文献求助10
4分钟前
勇往直前发布了新的文献求助10
4分钟前
共享精神应助MaxWong采纳,获得10
4分钟前
4分钟前
MaxWong发布了新的文献求助10
4分钟前
4分钟前
量子星尘发布了新的文献求助10
4分钟前
TOUHOUU完成签到 ,获得积分10
5分钟前
量子星尘发布了新的文献求助10
5分钟前
wodetaiyangLLL完成签到 ,获得积分10
5分钟前
高分求助中
Africanfuturism: African Imaginings of Other Times, Spaces, and Worlds 3000
Les Mantodea de Guyane: Insecta, Polyneoptera [The Mantids of French Guiana] 2000
Electron microscopy study of magnesium hydride (MgH2) for Hydrogen Storage 1000
Structural Equation Modeling of Multiple Rater Data 700
 Introduction to Comparative Public Administration Administrative Systems and Reforms in Europe, Third Edition 3rd edition 590
全球膝关节骨性关节炎市场研究报告 555
Exhibiting Chinese Art in Asia: Histories, Politics and Practices 540
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 物理 生物化学 纳米技术 计算机科学 化学工程 内科学 复合材料 物理化学 电极 遗传学 量子力学 基因 冶金 催化作用
热门帖子
关注 科研通微信公众号,转发送积分 3892461
求助须知:如何正确求助?哪些是违规求助? 3435216
关于积分的说明 10791581
捐赠科研通 3160172
什么是DOI,文献DOI怎么找? 1745408
邀请新用户注册赠送积分活动 842891
科研通“疑难数据库(出版商)”最低求助积分说明 786929