Nitrogen-doped carbon dots: a novel biosensing platform for selective norfloxacin detection and bioimaging

Incomplete metabolism and non-biodegradable nature of norfloxacin (NORx) lead to its persistent residues in the environment and food, potentially fostering the emergence of antibiotic resistance and posing a significant threat to public health. Hence, we developed a norfloxacin sensor employing hydrothermally synthesized N-doped carbon dots (N-Ch-CQDs) from chitosan and PEI demonstrated high sensitivity and specificity towards the antibiotic detection. The quantum yield of excitation-dependent emission of N-Ch-CQDs was effectively tuned from 4.6 to 21.5% by varying the concentration of PEI (5-15%). With the enhanced fluorescence in the presence of norfloxacin, N-Ch-CQDs exhibited a linear detection range of 20-1400 nM with a limit of detection (LoD) of 9.3 nM. The high biocompatibility of N-Ch-CQDs was confirmed in the