A fluorogenic ROS-triggered hydrogen sulfide donor for alleviating cerebral ischemia-reperfusion injury

Rationale: Cerebral ischemia-reperfusion injury is a severe neurovascular disease that urgently requires effective therapeutic interventions.Recently, hydrogen sulfide (H2S) has garnered significant attention as a potential treatment for stroke; however, the precise and targeted delivery of H2S remains a considerable challenge for its clinical application.Methods: We have developed HSDF-NH2, a novel H2S donor characterized by high selectivity, self-reporting capabilities, and the ability to penetrate the blood-brain barrier (BBB).Results: HSDF-NH2 effectively scavenges reactive oxygen species (ROS) while generating H2S, with emitted fluorescence facilitating the visualization and quantification of H2S release.This compound has demonstrated protective effects against cerebral ischemia-reperfusion (I/R) injury and contributes to the reconstruction of brain structure and function in a rat stroke model (tMCAO/R).Conclusion: As a ROS-responsive, self-reporting, and fluorescent H2S donor, HSDF-NH2 holds considerable promise for the treatment of ischemic diseases beyond stroke.