脱氧核酶
合理设计
合成生物学
纳米技术
DNA
化学
级联
计算机科学
计算生物学
生物系统
材料科学
生物
生物化学
色谱法
作者
Carl W. Brown,Matthew R. Lakin,Eli K. Horwitz,M. Leigh Fanning,Hannah E. West,Darko Stefanović,Steven W. Graves
标识
DOI:10.1002/anie.201402691
摘要
Signal propagation through enzyme cascades is a critical component of information processing in cellular systems. Although such systems have potential as biomolecular computing tools, rational design of synthetic protein networks remains infeasible. DNA strands with catalytic activity (DNAzymes) are an attractive alternative, enabling rational cascade design through predictable base-pair hybridization principles. Multi-layered DNAzyme signaling and logic cascades are now reported. Signaling between DNAzymes was achieved using a structured chimeric substrate (SCS) that releases a downstream activator after cleavage by an upstream DNAzyme. The SCS can be activated by various upstream DNAzymes, can be coupled to DNA strand-displacement devices, and is highly resistant to interference from background DNA. This work enables the rational design of synthetic DNAzyme regulatory networks, with potential applications in biomolecular computing, biodetection, and autonomous theranostics.
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