维生素E
维生素
显微解剖
化学
视网膜
气相色谱-质谱法
视黄醇
生育酚
质谱法
视网膜
多不饱和脂肪酸
色谱法
生物
生物化学
内科学
抗氧化剂
医学
脂肪酸
神经科学
基因
作者
Robert J. Stephens,D.S. Negi,Sarah Short,Frederik J.G.M. van Kuijk,Edward A. Dratz,David W. Thomas
标识
DOI:10.1016/0014-4835(88)90007-3
摘要
Vitamin E is thought to be important for protection of polyunsaturated fatty acids (PUFA) from oxidative damage. A microbiochemical procedure using microdissection and gas chromatography-mass spectrometry was developed to determine vitamin E distribution in ocular tissues in a rodent model, with the eventual goal of using it in a study of phototoxic degeneration of the retina, where PUFA oxidation is potentially the causal mechanism. Sample preparation was achieved by freeze-drying the retina followed by micro-dissection to obtain the desired structures for analysis. A deuterated alpha-tocopherol internal standard is added to the tissue sample before extraction and derivatization which are achieved in a single step. The data presented show the vitamin-E content in various structures of the retina, particularly the outer segments and retinal pigment epithelium (RPE); however, the vitamin E content of other ocular tissues is also included. Data were obtained from albino and pigmented rats receiving vitamin E-depleted, supplemented, and regular chow diets, and from rabbits and cats receiving regular chow diets formulated for each species. Within all dietary groups the highest concentration of vitamin E was located in the RPE followed by the outer segments of the photoreceptor cells. Other ocular tissues consistently contained lower amounts of vitamin E. Different tissues were depleted of vitamin E at different rates and this points out the importance of determining vitamin E levels in tissues of interest in studies on the consequences of dietary depletion.
科研通智能强力驱动
Strongly Powered by AbleSci AI