竞争性内源性RNA
小RNA
生物
非编码RNA
计算生物学
基因
遗传学
核糖核酸
基因表达
长非编码RNA
生物信息学
作者
Wenzhao Wang,Yanlin Su,Shi Tang,Hongfei Li,Wei Xie,Jianan Chen,Lin Shen,Xinda Pan,Bin Ning
出处
期刊:Aging
[Impact Journals, LLC]
日期:2019-04-18
卷期号:11 (8): 2352-2368
被引量:28
标识
DOI:10.18632/aging.101919
摘要
To systematically profile and characterize the noncoding RNA (ncRNA) expression pattern in the lesion epicenter of spinal tissues after traumatic spinal cord injury (TSCI) and predicted the structure and potential functions of the regulatory networks associated with these differentially expressed ncRNAs and mRNAs.A total of 498 circRNAs, 458 lncRNAs, 155 miRNAs and 1203 mRNAs were identified in TSCI mice models to be differentially expressed. The regulatory networks associated with these differentially expressed ncRNAs and mRNAs were constructed.We used RNA-Seq, Gene ontology (GO), KEGG pathway analysis and co-expression network analyses to profle the expression and regulation patterns of noncoding RNAs and mRNAs of mice models after TSCI. The findings were validated by quantitative real-time PCR (qRT-PCR) and Luciferase assay.noncoding RNAs might play important roles via the competing endogenous RNA regulation pattern after TSCI, further findings arising from this study will not only expand the understanding of potential ncRNA biomarkers but also help guide therapeutic strategies for TSCI.
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