平原的
胚芽
再生(生物学)
生物
细胞生物学
Wnt信号通路
RNA干扰
基因敲除
涡虫
基因沉默
干细胞
旁分泌信号
扁虫
细胞分化
信号转导
遗传学
核糖核酸
基因
生态学
受体
作者
Weiwei Wu,Shuo Liu,Hao Wu,Meishan Chen,Lili Gao,Baisuo Zhao,Baohua Liu,Qiuxiang Pang
标识
DOI:10.1016/j.ijbiomac.2022.04.095
摘要
Freshwater planarian Dugesia japonica is an excellent model organism for investigating stem cell behavior during regeneration. Despite studies showing that numerous genetic factors are involved in regeneration, much more research is required to fully understand the molecular mechanisms that orchestrate regeneration. In this study, we identified an evolutionarily conserved gene DjPtpn11(DjShp2). DjPtpn11 transcripts are expressed in neoblasts and some differentiated cells, with a high expression at the newly formed blastema. Its silencing by RNA interference (RNAi) affected anterior regeneration and inhibited the regeneration of posterior regions, including cholinergic and serotonergic neuron regeneration. In adult planarians, DjPtpn11 knockdown did not affect neoblast survival and proliferation but might prevent the stem cell migration and differentiation through ERK signaling. DjPtpn11 was demonstrated to be necessary for the anterior blastema cell differentiation partially via regulating ERK-DjMkpA activity. DjPtpn11 also influenced posterior specification via DjIslet, suggesting that DjPtpn11 may be involved in regulating the Wnt signaling pathway during the development of posterior blastema. Together, these data identified that DjPtpn11 is an essential modulator for the regeneration of planarians, and it may influence the appropriate differentiation of blastema cells.
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