化学
适体
检出限
拉曼光谱
色谱法
双金属片
互补DNA
表面增强拉曼光谱
罗丹明6G
胶体金
分子
分析化学(期刊)
纳米颗粒
纳米技术
拉曼散射
生物化学
分子生物学
金属
有机化学
物理
材料科学
基因
光学
生物
作者
Nuo Duan,Tangbin Yao,Changxin Li,Zhouping Wang,Shijia Wu
出处
期刊:Talanta
[Elsevier]
日期:2022-08-01
卷期号:245: 123445-123445
被引量:16
标识
DOI:10.1016/j.talanta.2022.123445
摘要
We designed a SERS-based method to detect β-lactoglobulin (β-Lg), a major allergen of milk, utilizing aptamers to specifically anchor β-Lg. The Au-Ag nanourchins with excellent Raman enhancement effects were prepared and modified with a β-Lg aptamer as the capture substrate. By taking advantage of the hybridization of aptamer and Raman reporter molecule (6-Carboxyl-X-Rhodamine: ROX)-labeled complementary DNA (cDNA), when ROX is close to Au-Ag nanourchins, the SERS signal is enhanced. In the presence of β-Lg, aptamer binding to β-Lg induces the dissociation of ROX-cDNA from Au-Ag nanourchins, thereby decreasing the SERS signal. Under optimal conditions, the present bioassay provides a wide detection range of 10 ng/mL to 1000 ng/mL with a detection limit of 0.07 ng/mL. In addition, the established SERS sensor can selectively and accurately identify β-Lg with recovery rates of 93.25-107.0% in milk samples. It provides great potential as a sensitive and reliable detection method against the growing threat of allergens.
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