毛花素
类黄酮
非生物胁迫
化学
类黄酮生物合成
生物合成
生物化学
干旱胁迫
脯氨酸
芒柄花素
植物
生物
食品科学
基因表达
酶
基因
抗氧化剂
转录组
氨基酸
大豆黄酮
染料木素
内分泌学
作者
Yinghui Chen,Bingzhen Li,Xin Jia,Shuying Sun,Youla Su,Guilin Chen
标识
DOI:10.1007/s12010-022-03883-y
摘要
Calycosin-7-O-β-D-glycoside (CG), as a flavonoid, plays an important role in the abiotic stress response of Astragalus membranaceus Bge. var. mongholicus (Bge.) Hsiao (A. mongholicus). CG is also an active ingredient in A. mongholicus with high medicinal value. However, the response mechanism of the CG biosynthetic pathway of drought stress is not clear. In this research, drought stress was inflicted upon A. mongholicus, and the variations in flavonoid metabolites and the correlating gene expression in CG biosynthesis were studied in roots, stems, and leaves of A. mongholicus by UHPLC-MRM-MS/MS and qRT-PCR. Drought stress reduced the dry weight and increased the content of malondialdehyde (MDA) and proline. Drought was beneficial to the accumulation of L-phenylalanine and 4-coumaric acid in leaves and promoted the accumulation of all target compounds in the roots, except calycosin. Overexpression of AmIOMT was observed in the leaves, but the content of formononetin which is the product of isoflavone O-methyltransferase (IOMT) catalysis was higher in stems than in leaves. This research aims to further understand the acclimation of abiotic stress and the regulation mechanism of flavonoid accumulation in A. mongholicus.
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