肠道菌群
拟杆菌
厚壁菌
卵清蛋白
生物
拟杆菌
微生物学
免疫学
普雷沃菌属
细菌
16S核糖体RNA
遗传学
免疫系统
作者
Yuanyuan He,Yang Luo,Qinwan Huang,Hongyun Zhou,Ming Qian,Zhonghong Guan,Qianwei Liu,Qiong Zhao
出处
期刊:Research Square - Research Square
日期:2020-07-08
标识
DOI:10.21203/rs.3.rs-38586/v1
摘要
Abstract Background: To investigate the effects of Xiaoning liquid on gut microbiota in mouse during asthma. Methods: A total of 60 mice were randomly and averagely assigned to healthy control group, control group, budesonide group, and Xiaoning liquid group. The later three groups were used to establish an Ovalbumin (OVA) asthma model. The intestinal bacterial communities were compared among groups using 16S rRNA gene amplification. Analyzing the structure of gut microbiota with OTU analysis, Shannon–Wiener, PCA, PCOA, etc. 16s rDNA high- throughput sequencing. Results: The abundance and diversity of the gut microbiota in asthmatic mice increased, most obviously in the control group. The Bacteroidetes and Firmicutes levels increased in all asthmatic mice. The level of Bacteroides increased most obviously, making Bacteroide s a useful marker of gut microbiota changes in asthmatic mice. The levels of Proteobacterium , Deferribacteraceae and Mucispirillum dropped significantly in the Xiaoning liquid group. Conclusions: Xiaoning liquid can reduce the species and numbers of pathogenic bacteria and restored the intestinal microecology of asthmatic mice. Xiaoning liquid has a positive effect on the function of gut microbiota.
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