孢子
双癸酸
过氧化氢
枯草芽孢杆菌
发芽
微生物学
化学
内孢子
芽孢杆菌(形态)
过氧化物
芽孢杆菌目
生物化学
生物
细菌
植物
有机化学
遗传学
作者
Elizabeth Melly,Ann E. Cowan,Peter Setlow
标识
DOI:10.1046/j.1365-2672.2002.01687.x
摘要
Aims: To determine the mechanism of killing of Bacillus subtilis spores by hydrogen peroxide. Methods and Results: Killing of spores of B. subtilis with hydrogen peroxide caused no release of dipicolinic acid (DPA) and hydrogen peroxide-killed spores were not appreciably sensitized for DPA release upon a subsequent heat treatment. Hydrogen peroxide-killed spores appeared to initiate germination normally, released DPA and hydrolysed significant amounts of their cortex. However, the germinated killed spores did not swell, did not accumulate ATP or reduced flavin mononucleotide and the cores of these germinated spores were not accessible to nucleic acid stains. Conclusions: These data indicate that treatment with hydrogen peroxide results in spores in which the core cannot swell properly during spore germination. Significance and Impact of the Study: The results provide further information on the mechanism of killing of spores of Bacillus species by hydrogen peroxide.
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