化学
色谱法
药代动力学
最大值
四极飞行时间
普罗托品
分析物
口服
质谱法
飞行时间质谱
分辨率(逻辑)
药理学
串联质谱法
生物碱
立体化学
有机化学
医学
人工智能
计算机科学
离子
电离
作者
Ye Tao,Haiyu Xu,Shanshan Wang,Bing Wang,Yingchun Zhang,Weihao Wang,Bin Huang,Hongwei Wu,Defeng Li,Yi Zhang,Xuefeng Xiao,Yubo Li,Hongjun Yang,Luqi Huang
标识
DOI:10.1016/j.jchromb.2013.07.015
摘要
Yuanhu Zhitong prescription (YZP) is well known for its analgesic effect. However, its multiple bioactive components in vivo remain unclear. In this paper, a rapid resolution liquid chromatography/quadrupole time-of-flight (RRLC-ESI-Q/TOF) was employed to identify the bioactive components and partial metabolites after oral administration of YZP extracts. Meanwhile, a RRLC-ESI-Q/TOF method was established and validated for the simultaneous quantification of protopine, α-allocryptopine, tetrahydropalmatine, corydaline, tetrahyberberine and byakangelicin in rat plasma and applied for their pharmacokinetic research. The results showed that twenty-one bioactive components of YZP were absorbed into the blood circulation and seventeen components were detected in cerebrospinal fluid (CSF). Moreover, the kinetic profiles of six analytes were obtained and the results suggested that the six analytes peaked between 3.5 and 5.0h and Cmax ranged from 214.6 to 858.3. The works could provide key information for identification of bioactive constituents and understanding the metabolism as well as pharmacological actions for YZP.
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