Investigation of transposons,insertion sequences and integron in multidrug-resistant acinetobacter baumannii

OBJECTIVE To investigate the distribution of genetic markers of mobile genetic elements: transposons,insertion sequences and integron in multidrug-resistant Acinetobacter baumannii.METHODS From Jan to Dec 2008,20 strains of multidrug-resistant A.baumannii isolated from sputum were collected from a hospital of a county-level city.Then,5 kinds of genetic markers of mobile genetic elements(tnpU,tnp513,IS26,ISaba1,intⅠ1) were analyzed by PCR.RESULTS All 5 kinds of genetic markers of mobile genetic elements were detected,and 15 strains(75.0%),15 strains(75.0%),18 strains(90.0%),17 strains(85.0%),15 strains(75.0%)were detected to carry tnpU,tnp513,IS26,ISaba1,intⅠ1,respectively.CONCLUSION The high rate of drug resitance of MDR-ABA to beta-lactams,aminoglycosides,quinolones is associated to 5 kinds of genetic markers of mobile genetic elements detected in 20 strains of multidrug-resistant A.baumannii.