Content of active constituents in Pogostemon cablin by tissue culture and cutting propagation

Objective To determine and compare the content of patchouli alcohol and pogostone in Pogostemon cablin tissue culture and cutting propagation,and to offer reference for tissue culture condition and quality evaluation.Methods With GC method,content of patchouli alcohol and pogostone was determined in 6 batches of medicinal herbs by tissue culture and in another 6 batches by cutting propagation.Results The content of patchouli alcohol and pogostone in the 2 medicinal herbs had no significant difference.Conclusion Tissue culture can be used as the propagation method and could ensure the quality of Pogostemon cablin.