Study on the Preparation of Xylooligosacchrides from Corncob with Acid-xylanase Hydrolysis

The preparation process of xylooligosacchrides from corncob with acid-xylanase hydrolysis was systematically studied.The primary treatment method was that the corncob was soaked in water at 60 ℃ for 12 hours,and then filtrated and dryed.The acidic primary hydrolysis was steamed at 120 ℃ for 1 hour(10 g corncob in 60 ml of 2.0 g/L H2SO4).The yield of total sugar was 15.01% and DP 2.16.The conditions of enzymatic hydrolysis were pH 4.8,the amount of xylanase 40 IU/g corncob,and at 50 ℃ for 4 hours.The yield of total sugar was 20.32% and DP 1.74.The purity of xylooligosacchrides was 62.37%.