Determination of vanillin, methyl vanillin and ethyl vanillin in milk powder

An ultra performance liquid chromatography(UPLC) method was developed for the simultaneous determination of vanillin, methyl vanillin and ethyl vanillin in milk powder. Samples were dissolved with water, purified with solid phase extraction column, separated with C18 column, and determined with ultra performance liquid chromatography. The results showed that there were good linear relationships between peak areas and concentrations of vanillin, methyl vanillin and ethyl vanillin in the range of 0.5~20 mg/L, with limits of detection of0.20 mg/L、0.25mg/L and 0.25 mg/L, respectively. The mean recoveries at spiked levels of 2.5~10.0 mg/L were in the range of 79.2%~102.7%, with the relative standard deviations(RSDs, n=6) of 1.85%- 4.11%. This method is sensitive and reliable, and could meet the requirements for simultaneous of vanillin, methyl vanillin and ethyl vanillin in milk powder.