Effects of proteasome inhibitor MG-132 on SHG-44 glioma cell in vitro

Objective To investigate the effects of proteasome inhibitor MG-132 on human glioma SHG-44 cell proliferation,cell apoptosis and cell cycle.Methods Human glioma SHG-44 cells were cultured with different concentrations of MG-132(5、10、20、50 μmol/L).Cell viability was determined by MTT assay at different cultured time points.Flow cytometry was used to detect cell apoptosis and cell cycle and AO/EB dye and HE method was used to detect SHG-44 cell apoptosis.Results The viability of SHG-44 cells of MG-132 group decreased significantly(P0.01),especially at 24 h.The cell proliferation OD values in MG-132(5、10、20、50 μmol/L) were significantly lower than those in normal control group(P0.01),presenting dose dependent.The obvious apoptosis subdiploid peak was detected 12 h after treated by 5 μmol/L of MG-132 with time and quantity effect correlation.The result of AO/EB and HE stain was basically same with that of flow cytometry.The cell expression percentages of G2/M phage were significantly higher,those of S phage were significantly lower(P0.01) in different dosages of MG-132 at 24 h than those in normal control groups(P0.01).Conclusions Proteasome inhibitor MG-132 could induce human glioma SHG-44 cell apoptosis,cell cycle arrest and proliferation inhibition.