Analysis of peripheral blood T cell subsets and Th1/Th2 cells in aplastic anemia

Objective To determine the cellular immunological abnormalities in aplastic anemia(AA). Methods ①The quantity of the subsets of T lymphocyte and CD8~+HLA-DR~+ cells in peripheral blood of AA patients at diagnosis were detected respectively by FACS.②Th1 and Th2 cells in peripheral blood mononuclear cells(PBMNC) of AA patients were counted by FACS. Results ①In aplastic anemia patients,the percentage of CD3~+CD4~+ cells,CD3~+CD8~+cells,CD8~+HLA-DR~+cells and the ratio of CD4~+/CD8~+ were (35.05±9.47)%,(36.29±6.81)%,(18.36±2.07)% and 1.0±0.33 respectively.In normal controls,they were (52.84±4.03)%,(24.69±3.90)%,(11.13±1.14)% and 2.14±0.24 respectively.The percentage of CD3~+CD4~+ cells and the ratio of CD4~+/CD8~+ of AA patients were significantly lower than that of controls;the percentage of CD3~+CD8~+ cells and CD8~+HLA-DR~+ cells were significantly higher than that of controls;while the percentage of CD3~+cells had no difference between AA patients and normal controls.②The percentage of Th1 cells and the ratio of Th1/Th2 of AA patients were significantly higher than that of controls,the percentage of Th2 cells had no difference between AA patients and normal controls.③The percentage of Th1 cells in AA patients were positively correlated with their CD3~+CD8~+cells and negatively correlated with their RC and ANC. Conclusion Disbalance of T lymphocyte subsets and increases in quantity of Th1 cells might be important for the development of bone marrow failure in aplastic anemia.