Objective To study the effect of pueraria isoflavones on some blood biochemical and antioxidant indices of swimming training rat blood. Methods Healthy male Sprague-Dawley rats were divided into resting groups,training group,and training groups with different dose of pueraria isoflavones.Blood biochemical indices like GOT,GPT,GLU, HHB and antioxidant indices including SOD and MDA were tested. Results 100.0 mg/kg and 200.0 mg/kg pueraria isoflavones could obviously increase the activity of SOD and the content of GLU and HHB(P0.05),meanwhile decrease the content of MDA the activity of GOT(P0.05). Conclusion Pueraria isoflavones on exercise training has obvious an- tioxidant effect in rats and can obviously improve the blood biochemical indexes.