Endogenous single cell expression of TS sense (TS) and its antisense (rTSα) mRNA, as well as HIF sense (HIF) and its antisense (aHIF) RNA

Copyright information:Taken from "RNA interference is not involved in natural antisense mediated regulation of gene expression in mammals"Genome Biology 2006;7(5):R38-R38.Published online 9 May 2006PMCID:PMC1779516. Real-time PCR primers were designed to span between the overlapping and non-overlapping regions. Expression of the low abundant TATA box binding protein (TBP) was also quantified to determine the sensitivity of the assay. All samples were normalized to β2M and the average results from 15 individual cells are plotted.