共焦显微镜
光学
共焦
显微镜
荧光相关光谱
材料科学
猝灭(荧光)
超分辨显微术
分辨率(逻辑)
光学显微镜
荧光
荧光光谱法
激发态
光谱学
荧光显微镜
显微镜
衍射
分析化学(期刊)
化学
薄层荧光显微镜
物理
原子物理学
扫描电子显微镜
计算机科学
人工智能
量子力学
色谱法
作者
Thomas A. Klar,Stefan Jakobs,Marcus Dyba,Alexander Egner,Stefan W. Hell
标识
DOI:10.1073/pnas.97.15.8206
摘要
The diffraction barrier responsible for a finite focal spot size and limited resolution in far-field fluorescence microscopy has been fundamentally broken. This is accomplished by quenching excited organic molecules at the rim of the focal spot through stimulated emission. Along the optic axis, the spot size was reduced by up to 6 times beyond the diffraction barrier. The simultaneous 2-fold improvement in the radial direction rendered a nearly spherical fluorescence spot with a diameter of 90-110 nm. The spot volume of down to 0.67 attoliters is 18 times smaller than that of confocal microscopy, thus making our results also relevant to three-dimensional photochemistry and single molecule spectroscopy. Images of live cells reveal greater details.
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