A Rapid, Sensitive Method for the Quantitation of N-Acetyl-S-(2-Hydroxyethyl)-l-Cysteine in Human Urine Using Isotope-Dilution HPLC-MS-MS

色谱法 同位素稀释 化学 尿 固相萃取 代谢物 检出限 液相色谱-质谱法中的离子抑制 萃取(化学) 人口 样品制备 衍生化 环氧乙烷 质谱法 串联质谱法 生物化学 人口学 社会学 有机化学 共聚物 聚合物
作者
Dana Boyd Barr,David L. Ashley
出处
期刊:Journal of Analytical Toxicology [Oxford University Press]
卷期号:22 (2): 96-104 被引量:20
标识
DOI:10.1093/jat/22.2.96
摘要

Because of increasing concern about exposure to carcinogens and other toxicants, reliable methods for biological monitoring of potentially exposed populations must be developed. For biological monitoring to be useful, appropriate biomarkers of exposures to xenobiotics must be identified, and sensitive, specific methods for quantifying the targeted biomarker must be developed. We have developed a sensitive and selective method for the analysis of N-acetyl-S-(2-hydroxyethyl)-L-cysteine (HEMA), urinary metabolite of at least three different known human carcinogens (vinyl chloride, ethylene oxide, and ethylene dibromide). The method uses strong anion-exchange solid-phase extraction and isotope-dilution high-performance liquid chromatography-tandem mass spectrometry. Our method is simple and is not labor intensive; the preparation time per sample is less than 10 min. Because urine samples vary in both their concentration and ion strength, intersample variability in HEMA recovery during the extraction is large. To overcome this inherent limitation, we use the isotope-dilution technique, which allows a complete correction for the extraction recovery for each sample. The limit of detection of the method is 0.68 microgram/L in a 1-mL urine sample with a coefficient of variation of 22% (determined by replicate analyses at both 4 and 11 micrograms/L) and an accuracy indistinguishable from 100%. Preliminary analyses of urine from a population with no known overt exposure to the parent toxicants show a frequency of detection of approximately 75%, which indicates that this method has the sensitivity to detect urinary HEMA derived from environmental exposure. We are currently using this method to establish a reference range of background exposure to these toxicants in the U.S. population.
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